TrulySafe™ DNA Stain – Protocol
- Melt agarose in your running buffer and cool to 50°C
- Add 5 μl of TrulySafe™ DNA Stain per 100 ml of cooled melted agarose solution
- Mix by swirling
- Pour your gel – keep from direct sunlight
For large fragments, there is no need to add TrulySafe™ DNA Stain to the running buffer. For smaller fragments, less than 400 bp, one can add TrulySafe™ DNA Stain to the running buffer (5 μl per 100 ml of buffer).
After the electrophoresis is finished, visualize on either a blue light transilluminator or a typical UV transilluminator.
TrulySafe™ DNA Stain can also be used as a post-run stain for either agarose or polyacrylamide gels. For this use, it is important to use a plastic container only. For optimal background, the gel thickness should be no more than 5 mm. Use 10 μl of TrulySafe™ DNA Stain per 100 ml of distilled water. Incubate the completed gel in this solution for 20 minutes (with periodic shaking). Rinse the gel with distilled water, then destain in 100 ml of distilled water for 20 minutes. The gel should now be suitable for photography. If need be, add another destain step. NOTE: The staining solution can be used several times, just keep it away from direct sunlight.