SingleStep™ Fast Stain – Overview + Protocol
SingleStep™ Fast Stain is a novel non-toxic and environmentally friendly stain for the rapid staining of proteins that have been separated using polyacrylamide gel electrophoresis.
SingleStep™ Fast Stain can stain proteins in as little as 15 min. And, no destain is required.
At room temperature, the bands will appear during the first 30 minutes and the intensity will increase over time and plateau after few hours. The minimum staining time may vary with gel concentration and composition.
For a staining process at room temperature, place the gel in a suitable container and add enough SingleStep™ Fast Stain to cover the gel and incubate, with gentle shaking, for 30 minutes.
Most highly concentrated protein bands should be visible within 15 to 30 min. If need be, incubation time can be extended at will.
No risk of over staining.
For small amounts of protein, for example, 1 ng, a 3 to 5 hours staining time is suggested.
No destain is required.
The gel percentage can influence the staining time. Higher percent acrylamide gels have smaller pore size and this can influence the stain time.
Note: Band intensity will increase with extended incubation time with a plateau reached after about 5 hours. These gels will be great for publication quality photographs.
SingleStep™ Fast Stain can be re-used several times (store the used stain in a separate container).