Ponceau S Staining Solution
IDSol Ponceau S stain comes in two product formats, liquid (500 ml) and powder. This is a 0.2% Ponceau S, 3% acetic acid solution. Ponceau S is used to determine electro-blot transfer efficiency by reversibly staining the membrane after blotting is complete.
Protocol: Place the electroblotted membrane in a small plastic dish and add enough Ponceau S solution to just cover the membrane. Incubate for 5 minutes at room temperature. Pour off the Ponceau S (can be re-used, store in separate bottle from the stock). Wash the membrane in 3% acetic acid until a clear background is achieved.
Cat # IDW06 is the liquid format and comes in a 500 ml bottle, ready-to-use.
Cat # IPW06 comes in powder format – 5 packets, each containing enough Ponceau S powder to make 100 ml of Ponceau S solution.