Cat # IDMW26: A 10 to 180 kDa Prestained Marker
Ready-to-use: supplied in a loading buffer for direct loading on gels. Easy to identify: includes the 28 & 75 kDa reference bands coupled with respectively a green and orange dye.
This Protein Ladder is a mixture of purified proteins covalently coupled to dyes that resolves to 10 bands after electrophoresis. Suitable for approximate molecular weight determination. For accurate molecular weight estimation of proteins, use unstained protein ladders.
Quality Control Tested in SDS-polyacrylamide gel electrophoresis and Western blotting
Storage Buffer Tris-HCl pH 6.8 (at 25°C), SDS, Triton X-100, DTT, Urea, Bromophenol blue and Glycerol
Content 0.1-0.2 mg/ml of each protein Storage Store at -20°C for 12 months
Stable at +4°C for 3 months Stable 20 days at RT once resuspended
Recommendations for Loading Load 5 µl per well, or 3 µl per well for blots
- Cat # IDMW26 (500 µl)